Linden blossom Freon extract morphological examination based on the model of adjuvant arthritis in rats

Abstract

Dmitry Demyanenko, Natalia Timchenko, Galina Belik, Maxim Evstigneev, Olesya Kudina, Yuri Stoletov, Maxim Zupanets, Dmitry Semeniv

Objective: Treatment of diseases of the rheumatoid arthritis provides use of nonsteroidal anti-inflammatory drugs and corticosteroids which have side effects and some drugs were discontinued. It is necessary to find biologically active substances of natural origin of sufficient anti-inflammatory activity which are safe compared to synthetic non-steroidal anti-inflammatory drugs. Blossom of different types of linden is widely used in medicine due to its diverse therapeutic action which can be sedative, expectorant, diaphoretic, diuretic, anxiolytic and antispasmodic. Methods: A powder mixture was formed from Linden blossom extract and inert filler (lactose) (Linden blossom powdered extract – LBPE). Anti-inflammatory properties of LBPE were studied on the model of adjuvant arthritis in white mongrel rats. Diclofenac sodium was selected as a comparison drug. Freund’s adjuvant, which was administered to animals subplantarly was used to reproduce adjuvant arthritis model. LBPE was intragastrically administered within the limits of the therapeutic and preventive regimen once a day from the first day after injection of adjuvant for 22 days in arbitrary effective dose of 30 mg / kg. Results: LBPE has a normalizing effect on condition of the overlying tissues, reducing the severity of synovitis, whereby no evidence of completely invaded and / or destroyed cartilage surfaces by the synovial membrane was registered. Morphometric parameters are better than in the control pathology, and the density of chondrocytes location reaches intact indices. Conclusions: Pronounced anti-inflammatory activity of LBPE are determined due to the model of adjuvant arthritis in rats. Under the influence of LBPE cell density indicator increases to intact values.