Association of Vitamin D Receptor Gene G>T and T>C Polymorphisms in Young Onset Diabetes Mellitus in a Bangladeshi Population

Abstract

Debashish Paul, Imran Khan, Omar Faruque , Naiyuum Choudhury, Zahid Hasan, Liaquat Ali

Background: Vitamin D has an important immunomodulatory property and vitamin D receptor (VDR) gene polymorphism has also been found to be associated with insulin secretory capacity and glucose intolerance. The present study was undertaken to determine genotype of VDR gene common variants in young onset diabetic subjects of Bangladesh to explore its association with diabetes and B cell secretory capacity. Methods: A total number of 94 young diabetic subjects and 92 healthy controls were recruited. Blood parameters were estimated by standard methods and DNA was extracted using QIAGEN Kit. VDR gene variants [G>T and T>C] were determined by PCR-RFLP using restriction endonuclease Apa1 and Taq1 respectively. Data were managed using Statistical Program for Social Science (SPSS). Results: C-peptide level was found to be significantly lower 3.297 (pT and T>C genome frequencies (wild, heterozygous and homozygous variants) were in the control (0.183, 0.516, 0.316 and 0.370, 0.522, 0.109 respectively) and (0.198, 0.484, 0.319 and 0.484, 0.418, 0.090 respectively) in young diabetes mellitus (YDM) which did not show significant association with YDM (p=0.906 and 0.288 respectively). Genotype frequency of the marker allele did not show significant association with glucose, C-peptide, HOMA-%S and IR either in controls or YDM subjects except the HOMA-%B shown to have relatively lower. Conclusions: VDR gene G>T and T>C polymorphic marker are not associated with diabetes mellitus in the young Bangladeshi diabetic patients, Insulin secretory dysfunction is main feature of pathogenesis of diabetes in the young. VDR gene G>T genotype is associated with higher secretary capacity .