ISSN 2146-832X
 

Original Research 


Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman.

Abstract
To assess the seasonal effects on water & osmotically-active ionic contents (Na+, K+ & Cl-) of skeletal muscles of the spiny-tailed lizard Uromastix hardwickii.
Fresh animals (adults) of both the sexes were used in all the experiments, and the gastrocnemius (skeletal) muscles were dissected out, reptilian buffer solution was used to keep dissected muscles moist & use during experimentation. The muscle sample solutions were prepared by heating/drying and incinerating/ashing the muscle tissues, treating with acid and deionized water respectively. These samples were then used for the estimation of Na+, K+ & Cl- ions. The water content was found as percent wet muscle weight.
The average mean values of water content and these osmotically active ionic contents were found to fall significantly(P<0.05) from peak winter towards peak summer & vice versa.
The seasonal effects of these parameters, studied for the first time in the skeletal muscle of Uromastix hardwickii, displayed changes as the animal passed from one seasonal phase to another. Hence seasonal changes are invariably associated with changes in environmental temperature, and definitely responsible to change the active behaviour and homeostasis of these animals from summer towards winter by variations in blood borne humoral and hormonal substances along with significant changes in osmolality and tissue water contents.

Key words: Colorimetric test (TPTZ method); Flame photometer; Osmolality parameters Na+, K+ & Cl-; Uromastix hardwickii; Water content


 
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How to Cite this Article
Pubmed Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. J Mol Pathophysiol. 2012; 1(1): 49-52. doi:10.5455/jmp.20120626014034


Web Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. http://www.jmolpat.com/?mno=21640 [Access: November 19, 2019]. doi:10.5455/jmp.20120626014034


AMA (American Medical Association) Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. J Mol Pathophysiol. 2012; 1(1): 49-52. doi:10.5455/jmp.20120626014034



Vancouver/ICMJE Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. J Mol Pathophysiol. (2012), [cited November 19, 2019]; 1(1): 49-52. doi:10.5455/jmp.20120626014034



Harvard Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman (2012) Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. J Mol Pathophysiol, 1 (1), 49-52. doi:10.5455/jmp.20120626014034



Turabian Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. 2012. Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. Journal of Molecular Pathophysiology, 1 (1), 49-52. doi:10.5455/jmp.20120626014034



Chicago Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. "Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii." Journal of Molecular Pathophysiology 1 (2012), 49-52. doi:10.5455/jmp.20120626014034



MLA (The Modern Language Association) Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman. "Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii." Journal of Molecular Pathophysiology 1.1 (2012), 49-52. Print. doi:10.5455/jmp.20120626014034



APA (American Psychological Association) Style

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman (2012) Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii. Journal of Molecular Pathophysiology, 1 (1), 49-52. doi:10.5455/jmp.20120626014034





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